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recombinant human bmp4  (R&D Systems)


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    R&D Systems recombinant human bmp4
    Recombinant Human Bmp4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+bmp4/Recombinant+Human+BMP-4+Protein/pm42014719-310-33-36
    Average 97 stars, based on 1142 article reviews
    recombinant human bmp4 - by Bioz Stars, 2026-09
    97/100 stars

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    Recombinant:

    Article Title: Generation and characterization of vestibular inner ear organoids from human pluripotent stem cells
    Article Snippet: .. Hydrochloric acid (HCl; Thermo Fisher Scientific, cat. no. A144S-500) CRITICAL For reconstituting human recombinant BMP4 from R&D Systems. ..

    Article Title: Method for producing retinal tissue
    Article Snippet: .. At 3 days after the start of the suspension culture, gfCDM+KSR medium not containing a ROCK inhibitor and SAG but containing human recombinant BMP4 (R&D Systems) (4.5 nM) was added by 50 μl per 1 well such that the final concentration of exogeneous human recombinant BMP4 was 1.5 nM (55 ng/ml) (start of step 3). ..

    Article Title: Activation-derepression synergy enables a bHLH network to coordinate a signal-specific fate response.
    Article Snippet: Cultures were passaged using Accutase (Millipore Sigma Catalog #SF003) every 2 days or cryopreserved in liquid nitrogen in basal medium supplemented with 10% DMSO. .. For LIF/BMP treatments, growth medium was replaced with neural basal medium typically containing 20 ng/mL recombinant BMP4 (R&D Systems, Catalog #314-BP) and/or 80 ng/mL recombinant LIF (R&D Systems, Catalog #8878-LF). ..

    Article Title: Unveiling the signal valve specifically tuning the TGF-β1 suppression of osteogenesis: mediation through a SMAD1-SMAD2 complex
    Article Snippet: .. Recombinant BMP4 (314-BP), activin A (338-AC) and TGF-β1 (240-B) were obtained from R&D Systems. .. Recombinant insulin (91077 C) was obtained from Merck.

    Article Title: Primary Cilia Are Required for Efficient BMP Signaling in Traumatic Heterotopic Ossification.
    Article Snippet: Transduction efficiency was assessed using a fluorescence microscope (EVOS M5000 Imaging System; Invitrogen, Thermo Fisher Scientific, Carlsbad, CA, USA). .. Following transduction, Ift88deficient cells were serum starved for 48 h and subsequently treated with recombinant BMP4 (50 ng/mL; R&D Systems, Minneapolis, MN, USA) for 1 h. Cells were then fixed in cold methanol for 30 min at −20 ◦C and permeabilized with 0.1% Triton X-100 for 10 min at room temperature. .. After blocking with 3% bovine serum albumin (BSA) for 2 h, cells were incubated with primary antibodies at 4 ◦C overnight, followed by incubation with fluorophore-conjugated secondary antibodies (Goat anti-rabbit IgG Alexa Fluor 647 (Cat. No. A21246, Invitrogen, Carlsbad, CA, USA) and goat anti-mouse IgG Alexa Fluor 488 (Cat. No. A10667, Invitrogen, Carlsbad, CA, USA) for 2 h at room temperature.

    Article Title: Method for producing retinal tissue
    Article Snippet: .. On day 3 from the start of the suspension culture (i.e., 3 days after the start of the suspension culture), gfCDM+KSR medium not containing a ROCK inhibitor but containing human recombinant BMP4 (R&D Systems) (4.5 nM) was added by 50 μl per 1 well such that the final concentration of exogeneous human recombinant BMP4 was 1.5 nM (55 ng/ml) (start of step 3). .. On day 6 (i.e., 6 days after the start of the suspension culture), on day 9 (9 days later), on day 12 (12 days later), on day 15 (15 days later) from the start of the suspension culture, a half amount of the medium was changed to a gfCDM+KSR medium not containing ROCK inhibitor or human recombinant BMP4, on day 18 from the start of suspension culture (18 days after the start of suspension culture), the cell aggregates were transferred to a 90 mm culture dish (petri dish for suspension culture, SUMITOMO BAKELITE CO., LTD.) containing 15 ml of a DMEM/F12+N2 medium (DMEM/F-12, GlutaMAX (Life Technologies), 1×N2 supplement (Life Technologies), 100 U/ml penicillin-100 μg/ml streptomycin) and continuously cultured in suspension under 37° C., 5% CO2 conditions.

    Article Title: Unveiling the signal valve specifically tuning the TGF-β1 suppression of osteogenesis: mediation through a SMAD1-SMAD2 complex.
    Article Snippet: .. Recombinant BMP4 (314-BP), activin A (338-AC) and TGF-β1 (240-B) were obtained from R&D Systems. .. Recombinant insulin (91077 C) was obtained from Merck.

    Article Title: Primary Cilia Are Required for Efficient BMP Signaling in Traumatic Heterotopic Ossification
    Article Snippet: Transduction efficiency was assessed using a fluorescence microscope (EVOS M5000 Imaging System; Invitrogen, Thermo Fisher Scientific, Carlsbad, CA, USA). .. Following transduction, Ift88 -deficient cells were serum starved for 48 h and subsequently treated with recombinant BMP4 (50 ng/mL; R&D Systems, Minneapolis, MN, USA) for 1 h. Cells were then fixed in cold methanol for 30 min at −20 °C and permeabilized with 0.1% Triton X-100 for 10 min at room temperature. .. After blocking with 3% bovine serum albumin (BSA) for 2 h, cells were incubated with primary antibodies at 4 °C overnight, followed by incubation with fluorophore-conjugated secondary antibodies (Goat anti-rabbit IgG Alexa Fluor 647 (Cat. No. A21246, Invitrogen, Carlsbad, CA, USA) and goat anti-mouse IgG Alexa Fluor 488 (Cat. No. A10667, Invitrogen, Carlsbad, CA, USA) for 2 h at room temperature.

    Suspension:

    Article Title: Method for producing retinal tissue
    Article Snippet: .. At 3 days after the start of the suspension culture, gfCDM+KSR medium not containing a ROCK inhibitor and SAG but containing human recombinant BMP4 (R&D Systems) (4.5 nM) was added by 50 μl per 1 well such that the final concentration of exogeneous human recombinant BMP4 was 1.5 nM (55 ng/ml) (start of step 3). ..

    Article Title: Method for producing retinal tissue
    Article Snippet: .. On day 3 from the start of the suspension culture (i.e., 3 days after the start of the suspension culture), gfCDM+KSR medium not containing a ROCK inhibitor but containing human recombinant BMP4 (R&D Systems) (4.5 nM) was added by 50 μl per 1 well such that the final concentration of exogeneous human recombinant BMP4 was 1.5 nM (55 ng/ml) (start of step 3). .. On day 6 (i.e., 6 days after the start of the suspension culture), on day 9 (9 days later), on day 12 (12 days later), on day 15 (15 days later) from the start of the suspension culture, a half amount of the medium was changed to a gfCDM+KSR medium not containing ROCK inhibitor or human recombinant BMP4, on day 18 from the start of suspension culture (18 days after the start of suspension culture), the cell aggregates were transferred to a 90 mm culture dish (petri dish for suspension culture, SUMITOMO BAKELITE CO., LTD.) containing 15 ml of a DMEM/F12+N2 medium (DMEM/F-12, GlutaMAX (Life Technologies), 1×N2 supplement (Life Technologies), 100 U/ml penicillin-100 μg/ml streptomycin) and continuously cultured in suspension under 37° C., 5% CO2 conditions.

    Concentration Assay:

    Article Title: Method for producing retinal tissue
    Article Snippet: .. At 3 days after the start of the suspension culture, gfCDM+KSR medium not containing a ROCK inhibitor and SAG but containing human recombinant BMP4 (R&D Systems) (4.5 nM) was added by 50 μl per 1 well such that the final concentration of exogeneous human recombinant BMP4 was 1.5 nM (55 ng/ml) (start of step 3). ..

    Article Title: Method for producing retinal tissue
    Article Snippet: .. On day 3 from the start of the suspension culture (i.e., 3 days after the start of the suspension culture), gfCDM+KSR medium not containing a ROCK inhibitor but containing human recombinant BMP4 (R&D Systems) (4.5 nM) was added by 50 μl per 1 well such that the final concentration of exogeneous human recombinant BMP4 was 1.5 nM (55 ng/ml) (start of step 3). .. On day 6 (i.e., 6 days after the start of the suspension culture), on day 9 (9 days later), on day 12 (12 days later), on day 15 (15 days later) from the start of the suspension culture, a half amount of the medium was changed to a gfCDM+KSR medium not containing ROCK inhibitor or human recombinant BMP4, on day 18 from the start of suspension culture (18 days after the start of suspension culture), the cell aggregates were transferred to a 90 mm culture dish (petri dish for suspension culture, SUMITOMO BAKELITE CO., LTD.) containing 15 ml of a DMEM/F12+N2 medium (DMEM/F-12, GlutaMAX (Life Technologies), 1×N2 supplement (Life Technologies), 100 U/ml penicillin-100 μg/ml streptomycin) and continuously cultured in suspension under 37° C., 5% CO2 conditions.

    Transduction:

    Article Title: Primary Cilia Are Required for Efficient BMP Signaling in Traumatic Heterotopic Ossification.
    Article Snippet: Transduction efficiency was assessed using a fluorescence microscope (EVOS M5000 Imaging System; Invitrogen, Thermo Fisher Scientific, Carlsbad, CA, USA). .. Following transduction, Ift88deficient cells were serum starved for 48 h and subsequently treated with recombinant BMP4 (50 ng/mL; R&D Systems, Minneapolis, MN, USA) for 1 h. Cells were then fixed in cold methanol for 30 min at −20 ◦C and permeabilized with 0.1% Triton X-100 for 10 min at room temperature. .. After blocking with 3% bovine serum albumin (BSA) for 2 h, cells were incubated with primary antibodies at 4 ◦C overnight, followed by incubation with fluorophore-conjugated secondary antibodies (Goat anti-rabbit IgG Alexa Fluor 647 (Cat. No. A21246, Invitrogen, Carlsbad, CA, USA) and goat anti-mouse IgG Alexa Fluor 488 (Cat. No. A10667, Invitrogen, Carlsbad, CA, USA) for 2 h at room temperature.

    Article Title: Primary Cilia Are Required for Efficient BMP Signaling in Traumatic Heterotopic Ossification
    Article Snippet: Transduction efficiency was assessed using a fluorescence microscope (EVOS M5000 Imaging System; Invitrogen, Thermo Fisher Scientific, Carlsbad, CA, USA). .. Following transduction, Ift88 -deficient cells were serum starved for 48 h and subsequently treated with recombinant BMP4 (50 ng/mL; R&D Systems, Minneapolis, MN, USA) for 1 h. Cells were then fixed in cold methanol for 30 min at −20 °C and permeabilized with 0.1% Triton X-100 for 10 min at room temperature. .. After blocking with 3% bovine serum albumin (BSA) for 2 h, cells were incubated with primary antibodies at 4 °C overnight, followed by incubation with fluorophore-conjugated secondary antibodies (Goat anti-rabbit IgG Alexa Fluor 647 (Cat. No. A21246, Invitrogen, Carlsbad, CA, USA) and goat anti-mouse IgG Alexa Fluor 488 (Cat. No. A10667, Invitrogen, Carlsbad, CA, USA) for 2 h at room temperature.



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